fadG [2018-02-06T09:38:43.000Z]

fatty acid degradation

locus: BSU14071

pI: 8.56

mw: 64.00 kDa

protein length: 576 aa

gene length: 1728 bp

function: fatty acid degradation

product: unknown

essential: no

synonyms: ykuG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

Gene

Coordinates: 1,479,137 → 1,480,867

The protein

Protein family

• fadG family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|5D159F09BFD98B58A7EDAD37A4DB6468A702D09A|YbfG]

Expression and Regulation

Operons

• genes: [gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]-[gene|0264C72F95D53F0211B52753DA37B4A8C0E982D2|fadG]-[gene|B46D407B116E358DFB6284466DE5926EB3719C53|ykzU]
  description: [Pubmed|17189250]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]

  regulation

  • induced in the presence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-A768 (ykuG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/768 NBRP B. subtilis, Japan]
• BKE14071 (Δ[gene|0264C72F95D53F0211B52753DA37B4A8C0E982D2|fadG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14071 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATTACCTTCCTCCC, downstream forward: _UP4_TAGGGGAAGACCTAGTAAAT
• BKK14071 (Δ[gene|0264C72F95D53F0211B52753DA37B4A8C0E982D2|fadG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14071 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATTACCTTCCTCCC, downstream forward: _UP4_TAGGGGAAGACCTAGTAAAT

References

• 17189250,17189250,17189250